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Spectroscopic studies of galactose oxidase.

作者信息

Whittaker J W

机构信息

Department of Chemistry, Carnegie-Mellon University, Pittsburgh, Pennsylvania 15213, USA.

出版信息

Methods Enzymol. 1995;258:262-77. doi: 10.1016/0076-6879(95)58051-4.

Abstract

A combination of powerful spectroscopic approaches is now available for detailed characterization of active sites in free radical metalloenzymes. As illustrated for galactose oxidase, a single approach such as EPR or absorption spectroscopy is not sufficient to characterize a sample completely, and only by combining results from complementary techniques can the information in each approach be used most effectively.

摘要

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