Mirkova E, Ivanova-Chemishanska L, Khinkova L, Antov G, Mukhtarova M
Probl Khig. 1995;20:146-62.
At the Preparatory Workshop of the Plant for Automobile Tires (PAT), Sofia, complex investigations were undertaken to reveal possible genotoxic exposure. The studies included chemical analyses for levels of identifiable human carcinogens in the occupational ambient air (benz(a)pyrene, mineral oils, 2-naphthylamine); special techniques--questionnaire investigations and cytogenetic analysis by cytokinesis-block micronucleus method in peripheral lymphocyte cultures from 23 workers in occupational groups at risk; and urinalysis for contents of mercapturic acids. An out-of-house control group of 13 nonexposed subjects was concurrently investigated. For contents of benz(a)pyrene and mineral oils exceeding 2.5 to 3.5 times the respective occupational environment MACs, evidence from cytogenetic analysis showed substantial, 4-fold, increase in indexes of genotoxic impairment (frequency of micronucleated-binucleated lymphocytes, number of micronuclei per 1000 binucleated lymphocytes) in the workers investigated. These results are an indicator of genotoxic exposure and point to an increased potential risk of cancer development in the group of workers studied.
在汽车轮胎厂筹备研讨会上，于索非亚开展了复杂调查以揭示可能的基因毒性暴露情况。这些研究包括对职业环境空气中可识别的人类致癌物水平（苯并(a)芘、矿物油、2-萘胺）进行化学分析；采用特殊技术——对来自23名处于职业风险组的工人外周淋巴细胞培养物通过胞质分裂阻断微核法进行问卷调查和细胞遗传学分析；以及对硫醚氨酸含量进行尿液分析。同时对13名未暴露的外部对照组受试者进行了调查。对于苯并(a)芘和矿物油含量超过各自职业环境最大允许浓度2.5至3.5倍的情况，细胞遗传学分析证据显示，所调查工人的基因毒性损伤指标（微核双核淋巴细胞频率、每1000个双核淋巴细胞中的微核数量）大幅增加了4倍。这些结果表明存在基因毒性暴露，并指出所研究工人群体患癌潜在风险增加。
