Smith S, Lindefors N, Hurd Y, Sharp T
University of Oxford, Department of Clinical Pharmacology, Radcliffe Infirmary, UK.
Psychopharmacology (Berl). 1995 Aug;120(3):333-40. doi: 10.1007/BF02311181.
The present study examined the effects of acute and repeated administration of electroconvulsive shock (ECS) on levels of D1 and D2 receptor mRNAs in the nucleus accumbens and striatum (caudate-putamen) of the rat. Quantitative in situ hybridisation with 35S-labelled oligonucleotide probes specific for D1 and D2 receptor mRNAs was utilised. Compared to controls, rats receiving a single ECS showed higher levels of both D1 and D2 receptor mRNAs in the nucleus accumbens 4 h, but not 24 h, after treatment. Similarly, rats receiving ECS repeatedly (five ECS in 10 days) also exhibited higher levels of D1 and D2 receptor mRNAs in the nucleus accumbens 4 h, but not 24 h, after the last treatment. The effects of single and repeated ECS treatment on dopamine receptor mRNA levels were localised to the caudal region of the nucleus accumbens. No statistically significant changes in mRNA levels were detected in the striatum of rats treated with either acute or repeated ECS. We discuss the possibility that increased expression of D1 and D2 receptors in the nucleus accumbens may be involved in the dopamine-enhancing properties of ECS detected in behavioural studies.
本研究检测了急性和重复给予电惊厥休克（ECS）对大鼠伏隔核和纹状体（尾状核 - 壳核）中D1和D2受体mRNA水平的影响。采用了与D1和D2受体mRNA特异的35S标记寡核苷酸探针进行定量原位杂交。与对照组相比，接受单次ECS的大鼠在治疗后4小时而非24小时，伏隔核中D1和D2受体mRNA水平均较高。同样，重复接受ECS（10天内5次ECS）的大鼠在最后一次治疗后4小时而非24小时，伏隔核中D1和D2受体mRNA水平也较高。单次和重复ECS治疗对多巴胺受体mRNA水平的影响局限于伏隔核的尾部区域。在接受急性或重复ECS治疗的大鼠纹状体中，未检测到mRNA水平有统计学意义的变化。我们讨论了伏隔核中D1和D2受体表达增加可能与行为学研究中检测到的ECS增强多巴胺的特性有关的可能性。
