Uozumi J, Koikawa Y, Yasumasu T, Ueda T, Kumazawa J
Department of Urology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Res Exp Med (Berl). 1995;195(4):231-5. doi: 10.1007/BF02576792.
To investigate the protective action of methylprednisolone against cisplatin nephrotoxicity, the effect of in vivo pretreatment with methylprednisolone on the cisplatin-induced reduction in p-aminohippurate (PAH) accumulation and gluconeogenesis was examined using renal cortical slices prepared from Sprague-Dawley rats. The PAH accumulation in the kidney slices prepared from methylprednisolone-pretreated rats was significantly reduced following in vitro incubation with 2 mM cisplatin, to a degree equal to that observed in the slices prepared from untreated rats. However, the inhibitory effect of cisplatin on gluconeogenesis in the renal cortical slices obtained from methylprednisolone-pretreated rats was significantly smaller than that seen in the slices from untreated rats. Our present studies suggest that in vivo pretreatment with methylprednisolone may contribute to its protective effect against cisplatin nephrotoxicity through the process of gluconeogenesis in renal epithelial cells.
为研究甲泼尼龙对顺铂肾毒性的保护作用，使用从Sprague-Dawley大鼠制备的肾皮质切片，检测了甲泼尼龙体内预处理对顺铂诱导的对氨基马尿酸（PAH）蓄积减少和糖异生的影响。在用2 mM顺铂进行体外孵育后，从甲泼尼龙预处理大鼠制备的肾切片中PAH蓄积显著降低，降低程度与从未经处理大鼠制备的切片中观察到的程度相当。然而，顺铂对从甲泼尼龙预处理大鼠获得的肾皮质切片中糖异生的抑制作用明显小于从未经处理大鼠的切片中观察到的抑制作用。我们目前的研究表明，甲泼尼龙体内预处理可能通过肾上皮细胞糖异生过程对顺铂肾毒性发挥保护作用。
