Xia J Q, Lofstedt R M, Yason C V, Kibenge F S
Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada.
Res Vet Sci. 1995 Sep;59(2):183-5. doi: 10.1016/0034-5288(95)90058-6.
Two 18-month-old bovine herpesvirus 1 (BHV1)-seronegative bulls were inoculated experimentally with BHV1 via their prepuces. Semen collected at intervals was examined by optimised virus isolation, dot-blot hybridisation and the polymerase chain reaction (PCR) for detection of BHV1, and the infection was monitored serologically by using a virus neutralisation test. Antibodies were first detected 10 days after inoculation and were still present 40 days after inoculation. Semen collected from four to 40 days after inoculation was positive by PCR with Southern blot hybridisation whereas only the semen collected on day 4 was positive by dot-blot hybridisation, virus isolation and PCR with ethidium bromide staining. These results indicate that the bulls started to shed the virus in semen before they developed any detectable antibody. PCR with Southern blot hybridisation was the most sensitive of the three methods and detected virus for the longest period.
两头18月龄的牛疱疹病毒1型（BHV1）血清阴性公牛通过包皮进行了BHV1的实验性接种。定期采集精液，通过优化的病毒分离、斑点杂交和聚合酶链反应（PCR）检测BHV1，并使用病毒中和试验进行血清学监测感染情况。接种后10天首次检测到抗体，接种后40天抗体仍然存在。接种后4至40天采集的精液经Southern印迹杂交PCR检测呈阳性，而仅接种后第4天采集的精液经斑点杂交、病毒分离和溴化乙锭染色PCR检测呈阳性。这些结果表明，公牛在产生任何可检测到的抗体之前就开始在精液中排出病毒。Southern印迹杂交PCR是三种方法中最敏感的，检测病毒的时间最长。
