de Gee A L, Wagter L H, Hage J J
Animal Health Service, Drachten, The Netherlands.
Vet Microbiol. 1996 Nov;53(1-2):163-8. doi: 10.1016/s0378-1135(96)01244-8.
Nasal swabs from two bulls at an artificial insemination (AI) station were submitted to our laboratory. The animals showed clinical signs of Infectious bovine rhinotracheitis (IBR), although the station was supposedly free of bovine herpesvirus 1 (BHV1). DNA of BHV1 was detected using a polymerase chain reaction (PCR). Subsequently nasal swabs from 100 animals that could have been in contact were submitted. BHV1 DNA was detected in swabs from 23 animals. Using the PCR, BHV1 could only be detected in a limited number of semen samples over a period of two months prior to the outbreak or two months after the outbreak. Also, not all animals that shed BHV1 from the nose harboured detectable BHV1 in the semen. Finally BHV1 was detected in the semen of one bull, approximately six weeks before seroconversion. Presently the PCR is being used as a means of quality control of fresh semen from bulls that are seropositive for BHV1. We are able to produce a result within 6 h after the semen samples have been submitted, allowing the AI-station manager to take measures before semen distribution in the event of a positive reaction. So far 11 out of 318 samples were shown to contain BHV1 DNA. In order to be able to interpret these results an interlaboratory comparative study is proposed. In countries endemically infected with BHV1 the PCR can be a cost-effective method to minimize the risk of transmitting virus by semen.
一家人工授精（AI）站的两头公牛的鼻拭子被送到了我们实验室。这些动物表现出传染性牛鼻气管炎（IBR）的临床症状，尽管该站据推测没有牛疱疹病毒1型（BHV1）。使用聚合酶链反应（PCR）检测到了BHV1的DNA。随后，提交了100头可能有过接触的动物的鼻拭子。在23头动物的拭子中检测到了BHV1 DNA。在疫情爆发前两个月或爆发后两个月的时间里，使用PCR仅在有限数量的精液样本中检测到了BHV1。而且，并非所有从鼻腔排出BHV1的动物在精液中都含有可检测到的BHV1。最后，在一头公牛的精液中检测到了BHV1，大约在血清转化前六周。目前，PCR被用作对BHV1血清呈阳性的公牛新鲜精液进行质量控制的一种手段。精液样本提交后6小时内我们就能得出结果，这样如果反应呈阳性，人工授精站经理就能在精液分发前采取措施。到目前为止，318个样本中有11个被证明含有BHV1 DNA。为了能够解读这些结果，建议开展一项实验室间的比较研究。在BHV1地方流行感染的国家，PCR可以是一种具有成本效益的方法，以尽量降低通过精液传播病毒的风险。
