Pialarissi C S, Nitrini S M
Instituto Adolfo Lutz, São Paulo, Brasil.
Rev Saude Publica. 1995 Apr;29(2):115-9. doi: 10.1590/s0034-89101995000200005.
Comparisons were made among the erythroimmunoadsorption by capture (C-EIA), the immunoenzymatic (ELISA) and passive haemaglutination (P.H.A.) tests used in the diagnosis of neurocysticercosis. The three tests, two of them routine by in diagnosis, and the third one (C-EIA) recently, standardised were compared for the detection of anti-Cysticercus cellulosae antibodies. In the three reactions the antigen utilized was raw saline extract (R.S.E) with a yield of 0.1, 1 and 10 micrograms protein/cavity for the C-EIA, ELISA and P.H.A tests, respectively. In a group of 58 patients with neurocysticercosis, the sensitivity to the ELISA, C-EIA and P.H.A tests was of 98.2%, 84.5% and 77.2%, respectively. In the control group of 85 individuals, healthy or with encephalopathies other than neurocysticercosis, the sensitivity was of 98.27%, 84.48% and 79.63%, respectively, and the specificity was of 94.1%, 95.3% and 91.8%. This order of choice should be followed, depending on the resources available.
对用于神经囊尾蚴病诊断的捕获红细胞免疫吸附法(C-EIA)、免疫酶法(ELISA)和被动血凝试验(P.H.A.)进行了比较。这三种检测方法,其中两种是诊断中的常规方法,第三种(C-EIA)是最近标准化的方法,用于检测抗猪囊尾蚴抗体。在这三种反应中,所使用的抗原均为生理盐水粗提物(R.S.E),C-EIA、ELISA和P.H.A.试验中每腔的蛋白产量分别为0.1、1和10微克。在一组58例神经囊尾蚴病患者中,ELISA、C-EIA和P.H.A.试验的敏感性分别为98.2%、84.5%和77.2%。在85名健康或患有除神经囊尾蚴病以外的脑病的个体组成的对照组中,敏感性分别为98.27%、84.48%和79.63%,特异性分别为94.1%、95.3%和91.8%。应根据可用资源遵循这种选择顺序。