Pialarissi C S, Nitrini S M
Instituto Adolfo Lutz, Sã0 Paulo, Brasil.
Rev Saude Publica. 1994 Apr;28(2):116-20. doi: 10.1590/s0034-89101994000200004.
The capture erytoimmunoadsorption (C-EIA) test was standardized for detection of Taenia solium cysticercus-IgG specific antibodies in cerebrospinal fluid (CSF) from patients with neurocysticercosis. For the C-EIA test performance a reagent for specific antibody detection was prepared using sheep's red blood cells (SRBC) in a concentration of 0.25% sensitized with crude saline extract antigen (SEA) obtained from Cysticercus cellulosae. The optimum concentration of SEA for SRBC sensitization was 40 micrograms/ml. The yield of SEA was 0.1 microgram protein/cavity. When 58 CSF samples from patients with neurocysticercosis were analysed the sensitivity of the test was found to be 84.5% and the confidence limit of 95% probability (CL95%) ranged from 75% to 94%. The specificity was 95.3% (CL 95% from 90.7% to 99.9%) when CSF samples from the control group were analysed. The C-EIA test was shown to be efficient for neurocysticercosis diagnosis and important for public health laboratories, because of its low cost, high reagent yield and case of use.
已对捕获红细胞免疫吸附（C-EIA）试验进行标准化，以检测神经囊尾蚴病患者脑脊液（CSF）中猪带绦虫囊尾蚴IgG特异性抗体。对于C-EIA试验性能，使用浓度为0.25%的绵羊红细胞（SRBC）制备用于特异性抗体检测的试剂，该红细胞用从猪囊尾蚴获得的粗盐水提取物抗原（SEA）致敏。用于SRBC致敏的SEA最佳浓度为40微克/毫升。SEA的产量为0.1微克蛋白质/腔。当分析来自神经囊尾蚴病患者的58份CSF样本时，发现该试验的灵敏度为84.5%，95%概率的置信限（CL95%）范围为75%至94%。当分析对照组的CSF样本时，特异性为95.3%（CL 95%为90.7%至99.9%）。C-EIA试验被证明对神经囊尾蚴病诊断有效，对公共卫生实验室很重要，因为其成本低、试剂产量高且使用方便。
