Perocco P, Colacci A, Bonora B, Grilli S
Istituto di Cancerologia, Universitá di Bologna, Italy.
Teratog Carcinog Mutagen. 1995;15(2):73-80. doi: 10.1002/tcm.1770150204.
The cytotoxic effects and the transforming properties of two fungicides, metalaxyl and zineb, whose mutagenic or carcinogenic activity has not been clarified yet, were analyzed in the in vitro BALB/c 3T3 cell transformation test both in the presence and in the absence of an exogenous metabolizing system. Zineb was completely detoxified when the exogenous metabolizing system was added to the target cells to increase their inherent metabolic capacity. Metalaxyl induced cell transformation at any assayed dosage, i.e., 500, 250, and 50 micrograms/ml, in the presence of bioactivation, and at the highest dosage (500 micrograms/ml) in the absence of bioactivation. The transforming effect was detectable only in the level-II transformation cultures and it was likely linked to the induction of additional cell proliferation which allowed obtaining the transformation amplification in these experimental conditions.
在体外BALB/c 3T3细胞转化试验中，分析了两种杀菌剂甲霜灵和代森锌的细胞毒性作用及转化特性，这两种杀菌剂的诱变或致癌活性尚未明确，试验分别在有和没有外源性代谢系统的情况下进行。当向靶细胞添加外源性代谢系统以提高其固有代谢能力时，代森锌被完全解毒。在有生物活化的情况下，甲霜灵在任何测定剂量（即500、250和50微克/毫升）下均可诱导细胞转化，在没有生物活化的情况下，在最高剂量（500微克/毫升）时也可诱导细胞转化。转化效应仅在II级转化培养物中可检测到，并且可能与额外细胞增殖的诱导有关，这使得在这些实验条件下能够实现转化扩增。
