Baĭnov N A, Korshunov V M, Tarabrina N P, Efimov B A, Ginodman G A
Zh Mikrobiol Epidemiol Immunobiol. 1995 Sep-Oct(5):80-4.
The standardization of all components ensures the optimum experimental conditions; for this reason the obtaining and maintenance of animals meeting GLP requirements is one of the development of scientific research in biology and medicine. The aim of the present work was the study of the possibility of using gnotobiological technology for obtaining animals with different microbial status of the intestine and their maintenance. Our investigations revealed that obtaining animals with different microbial status of the intestine and their maintenance under the conditions of total gnotobiological isolation (TGBI) prevented the contamination of the animals, thus facilitating the standardization of research procedures. Gnotobiological technology made it possible to create the optimum maintenance conditions and to ensure constant microbial status in animals, free from pathogenic flora. The maintenance to totally decontaminated mice under TGBI conditions ensured the total germ-free status in these animals for 9 days.
所有组件的标准化确保了最佳的实验条件；因此，获得并维持符合GLP要求的动物是生物学和医学科研发展的关键之一。本研究的目的是探讨使用悉生生物学技术获得肠道微生物状态不同的动物并对其进行饲养的可能性。我们的研究表明，在完全悉生生物学隔离（TGBI）条件下获得肠道微生物状态不同的动物并对其进行饲养，可防止动物受到污染，从而有助于研究程序的标准化。悉生生物学技术能够创造最佳的饲养条件，并确保动物体内微生物状态恒定，无致病菌群。在TGBI条件下对完全净化的小鼠进行饲养，确保了这些动物在9天内保持完全无菌状态。
