Muhle H, Bellmann B
Fraunhofer-Institut für Toxikologie und Aerosolforschung, Teilinstitut Inhalationstoxikologie und Aerosolforschung, Hannover, Germany.
Ann Occup Hyg. 1995 Oct;39(5):655-60. doi: 10.1016/0003-4878(94)00106-b.
Methods for the determination of biodurability of man-made vitreous fibres are reviewed. For mineral wools the first step was the preparation of respirable fibre fractions. Fibres were administered to rats by inhalation or by intratracheal instillation. After serial sacrifice their lungs were digested by low-temperature ashing or by hypochlorite. The total number of fibres per lung and the distributions of length and diameter were analysed by electron microscopy. This resulted in a bivariate distribution of fibres at the various sacrifice dates. If the logarithm of the number of fibres decreased approximately linearly with time after exposure then the elimination kinetics of fibres can be characterized by a half-time. The half-times were compared between various experiments with rats exposed to mineral wool samples. In summary good agreement was found for the elimination of fibres after long-term inhalation and intratracheal instillation whereas shorter half-times were found after short-term inhalation.
本文综述了测定人造玻璃纤维生物耐久性的方法。对于矿棉，第一步是制备可吸入纤维部分。通过吸入或气管内滴注将纤维给予大鼠。在连续处死大鼠后，用低温灰化或次氯酸盐消化其肺脏。通过电子显微镜分析每只肺中的纤维总数以及长度和直径分布。这导致在不同处死日期纤维的双变量分布。如果暴露后纤维数量的对数随时间近似呈线性下降，那么纤维的消除动力学可以用半衰期来表征。比较了暴露于矿棉样品的大鼠在不同实验中的半衰期。总之，长期吸入和气管内滴注后纤维的消除情况有很好的一致性，而短期吸入后半衰期较短。
