大肠杆菌细胞高效表达鱼类生长激素。
Highly efficient expression of fish growth hormone by Escherichia coli cells.
作者信息
Tsai H J, Lin K L, Kuo J C, Chen S W
机构信息
Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan.
出版信息
Appl Environ Microbiol. 1995 Nov;61(11):4116-9. doi: 10.1128/aem.61.11.4116-4119.1995.
Abstract
A PCR product encoding the mature segment of fish pregrowth hormone (pre-GH) was inserted into an Escherichia coli expression vector, pET, in which the ori site was replaced by that of pUC19. The yield of recombinant GH (rGH) was as high as 44 to 47% of total protein. This rGH was immunoreactive to GH antibody. After renaturation, rGH was used to inject fish with 0.1 microgram of rGH per g once every 2 weeks, and this resulted in increases in weight (65%), percent weight gain (165%), and length (22%) relative to those of an untreated control group at week 16 and onward.
摘要
将编码鱼类前生长激素（pre-GH）成熟片段的PCR产物插入到大肠杆菌表达载体pET中，该载体的ori位点被pUC19的ori位点取代。重组生长激素（rGH）的产量高达总蛋白的44%至47%。这种rGH与生长激素抗体具有免疫反应性。复性后，用rGH以每克鱼0.1微克的剂量每2周注射一次，结果在第16周及之后，相对于未处理的对照组，鱼的体重增加了65%，增重百分比增加了165%，体长增加了22%。
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