Rouabah-Sadaoui L, Marcel R
Laboratoire d'écophysiologie d'invertébrés du sol, université des sciences et technologies de Lille, Villeneuve-d'Ascq, France.
Reprod Nutr Dev. 1995;35(5):537-48.
Biochemical methods were used to analyze the glucidic and lipidic components of the clitellar epithelium and the cocoon's albumen in Eisenia fetida. In the clitellum, the sugar concentration increased dramatically (about 5-fold) during differentiation. The assays showed increases in several monosaccharides during puberty. In addition, glucose was prominent in immature clitella, whereas mannose was the main sugar in the mature ones. About 50% of the dry weight of recently deposited cocoons was carbohydrates. The sugar concentration decreased in the albumen as hatching approached. The amount of total lipids in the clitellum increased during maturation. The levels of neutral lipids, however varied very little. Phospholipids began at low levels at the beginning of differentiation, and increased dramatically thereafter. The amount of fatty acids in the clitellar epithelium reached a maximum during the submature stage. Large amounts of fatty acids were stored in the cocoon. Palmitic, stearic, oleic and vaccenic acids were preponderant in both the clitellum and cocoon.
采用生化方法分析了赤子爱胜蚓环带上皮和茧蛋白中的糖类和脂质成分。在环带中,分化过程中糖浓度急剧增加(约5倍)。分析表明,青春期期间几种单糖有所增加。此外,葡萄糖在未成熟的环带中占主导地位,而甘露糖是成熟环带中的主要糖类。刚产下的茧中约50%的干重为碳水化合物。随着孵化临近,茧蛋白中的糖浓度降低。环带中的总脂质含量在成熟过程中增加。然而,中性脂质的水平变化很小。磷脂在分化开始时含量较低,此后急剧增加。环带上皮中的脂肪酸含量在亚成熟阶段达到最大值。大量脂肪酸储存在茧中。棕榈酸、硬脂酸、油酸和反油酸在环带和茧中均占优势。