Melo-Abreu S, Battish S, Brown K, Tureck R, Heyner S, Blasco L
Department of Obstetrics and Gynecology, University of Pennsylvania Medical Center, Philadelphia 19104, USA.
Am J Reprod Immunol. 1995 Aug;34(2):116-9. doi: 10.1111/j.1600-0897.1995.tb00926.x.
To evaluate the reproducibility of the Immunobead Assay (IBA) on sperm samples before and after cryopreservation.
Sperm samples (fresh and post-thaw) from known antibody negative donors (N = 20) were evaluated for percent immunobead binding by IBA following incubation with known antibody-positive serum.
In both fresh and thawed negative samples, the mean sperm head binding was 0.5% +/- 0.5, the mean sperm tail binding was 2.0% +/- 2.0 and the mean sperm head-tail binding was 3.0% +/- 2.0 for IgG, IgA and IgM type antibodies, respectively. The same samples exposed to positive sera showed 40.0% +/- 10.0 mean head binding, 7.0% +/- 8.0 mean tail binding and 47.0% +/- 11.0 mean head-tail binding.
IBA is highly reproducible for detecting sperm antibodies in both fresh and cryopreserved/thawed samples of human spermatozoa.
评估免疫珠试验（IBA）在精子样本冷冻保存前后检测精子抗体的可重复性。
将来自已知抗体阴性供体（N = 20）的精子样本（新鲜样本和解冻后样本）与已知抗体阳性血清孵育后，通过IBA评估免疫珠结合百分比。
在新鲜和解冻后的阴性样本中，对于IgG、IgA和IgM型抗体，精子头部的平均结合率为0.5%±0.5，精子尾部的平均结合率为2.0%±2.0，精子头部与尾部的平均结合率为3.0%±2.0。暴露于阳性血清的相同样本显示，头部平均结合率为40.0%±10.0，尾部平均结合率为7.0%±8.0，头部与尾部平均结合率为47.0%±11.0。
IBA在检测人类精子新鲜样本以及冷冻保存/解冻样本中的精子抗体方面具有高度可重复性。
