Franco J G, Schimberni M, Stone S C
J Reprod Med. 1987 Mar;32(3):188-90.
Circulating sperm antibodies have been traditionally quantified by measuring sperm agglutination (SAT) and immobilization (SIT) in serum. Recently an indirect immunobead assay (IBA) for detecting these antibodies in serum has been reported. Immunobeads coated with rabbit antibody to one of the three major immunoglobulins (IgG, IgM and IgA) are used. The procedure involves mixing a Hepes washed sample of normal donor sperm with the individual sera followed by centrifugation and incubation with each type of immunobead preparation and observed for evidence of binding. Sera from 40 patients previously tested with SAT and SIT were compared in four groups. There was a good correlation between IgG-IBA and SIT and a poor correlation between IgG-IBA and SAT. The class of immunoglobulin detected most frequently was IgG.
传统上，循环精子抗体是通过检测血清中的精子凝集（SAT）和制动（SIT）来定量的。最近，有报道称一种用于检测血清中这些抗体的间接免疫珠试验（IBA）。使用包被有针对三种主要免疫球蛋白（IgG、IgM和IgA）之一的兔抗体的免疫珠。该程序包括将经Hepes洗涤的正常供体精子样本与个体血清混合，然后离心，并与每种类型的免疫珠制剂孵育，观察结合证据。将40名先前用SAT和SIT检测过的患者的血清分为四组进行比较。IgG-IBA与SIT之间具有良好的相关性，而IgG-IBA与SAT之间的相关性较差。检测到的最常见免疫球蛋白类别是IgG。
