Debek W, Gruca P, Chyczewski L, Gruca A
Department of Pediatric Surgery, Medical Academy of Białystok.
Rocz Akad Med Bialymst. 1995;40(1):129-37.
The activity of rat peritoneal cells were assessed by the phorbol mirystinian acetate (PMA)-induced luminol chemiluminescence (LCL). Results in control groups (0 - no manipulation, and I - carotid artery cannulation) were compared with those in the untreated hemorrhagic shock (group II), in the shock treated with the standard polyelectrolyte solution (PES) (group III), and in shock treated with PAF receptor-antagonist BN 52021 + PES (group IV). The maximal and the most rapid LCL was observed in the group treated with BN 52021 (group IV), while chemiluminescent response in the the untreated shock (group II) and in shock treated with PES was minimally expressed and late. The findings indicate for a rapid activation of peritoneal cells during ca 1 hour of hemorrhagic shock. This leads to exhausting their ability to the superoxide anion generation 15 minutes later. Peritoneal cells obtained from the group treated with the BN 52021 revealed a preserved ability to the respiratory burst. It can be concluded that BN 52021 effectively inhibits activation of the PC during hemorrhagic shock.
通过佛波酯（PMA）诱导的鲁米诺化学发光（LCL）评估大鼠腹腔细胞的活性。将对照组（0 - 无操作，I - 颈动脉插管）的结果与未治疗的失血性休克组（II组）、用标准聚电解质溶液（PES）治疗的休克组（III组）以及用PAF受体拮抗剂BN 52021 + PES治疗的休克组（IV组）的结果进行比较。在用BN 52021治疗的组（IV组）中观察到最大且最快的LCL，而未治疗的休克组（II组）和用PES治疗的休克组中的化学发光反应表达最低且出现延迟。研究结果表明，在失血性休克约1小时期间腹腔细胞迅速激活。这导致15分钟后它们产生超氧阴离子的能力耗尽。从用BN 52021治疗的组获得的腹腔细胞显示出保留的呼吸爆发能力。可以得出结论，BN 52021在失血性休克期间有效抑制腹腔细胞的激活。
