Ishimoda-Takagi T, Yamazaki K, Ando T, Minami H, Nagata A
Department of Biology, Tokyo Gakugei University, Japan.
Comp Biochem Physiol B Biochem Mol Biol. 1995 Nov;112(3):415-27. doi: 10.1016/0305-0491(95)00092-5.
Egg tropomyosins were prepared from four sea urchin species, Stronglyocentrotus intermedius, Anthocidaris crassispina, Hemicentrotus pulcherrimus and Pseudocentrotus depressus, and their molecular heterogeneity was investigated by electrophoresis and immunoblotting. The molecular heterogeneity of egg tropomyosins was species-specific, and two to four kinds of tropomyosin isoforms were detected, the apparent molecular weights of which were 29,000-32,000. The egg tropomyosin isoforms could be classified into two groups with muscle- and nonmuscle-type antigenicities in each species. No obvious difference in their cytological localization was observed immunocytochemically in S. intermedius and H. pulcherrimus.
从四种海胆，即中间球海胆、厚刺海胆、马粪海胆和扁球海胆中制备了卵原肌球蛋白，并通过电泳和免疫印迹研究了它们的分子异质性。卵原肌球蛋白的分子异质性具有物种特异性，检测到两到四种原肌球蛋白同工型，其表观分子量为29,000 - 32,000。在每个物种中，卵原肌球蛋白同工型可分为具有肌肉型和非肌肉型抗原性的两组。在中间球海胆和马粪海胆中，通过免疫细胞化学未观察到它们在细胞定位上有明显差异。
