Tanihara H, Ohira A, Takahashi M, Honda Y, Suzuki S
Doheny Eye Institute, Los Angeles, CA 90033, USA.
Curr Eye Res. 1995 Aug;14(8):727-30. doi: 10.3109/02713689508998501.
It is known that trabecular meshwork cells produce proteoglycans and that local production may be associated with aqueous outflow resistance. In an attempt to identify intraocular production of proteoglycan decorin in the anterior chamber angle of mammalian eyes, we conducted a Northern blot analysis and immunohistochemical studies. Northern blot analysis suggested gene expression of proteoglycan decorin in trabecular meshwork cells. Also, immunohistochemical studies using anti-decorin antibody demonstrated decorin-like immunoreactivity in the trabecular meshwork and around the Schlemm's canal. Our data demonstrate the presence of proteoglycan decorin in the outflow pathway, suggesting that decorin is a component of extracellular matrices in these regions and may be associated with outflow resistance.
已知小梁网细胞会产生蛋白聚糖，且局部产生可能与房水流出阻力有关。为了确定蛋白聚糖核心蛋白聚糖在哺乳动物眼前房角的眼内产生情况，我们进行了Northern印迹分析和免疫组织化学研究。Northern印迹分析表明小梁网细胞中有蛋白聚糖核心蛋白聚糖的基因表达。此外，使用抗核心蛋白聚糖抗体的免疫组织化学研究显示，小梁网和施莱姆管周围存在核心蛋白聚糖样免疫反应性。我们的数据证明流出途径中存在蛋白聚糖核心蛋白聚糖，表明核心蛋白聚糖是这些区域细胞外基质的一个组成部分，可能与流出阻力有关。
