Howard N S, Gomez J E, Ko C, Bishai W R
Department of Molecular Microbiology and Immunology, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, MD 21205, USA.
Gene. 1995 Dec 1;166(1):181-2. doi: 10.1016/0378-1119(95)00597-x.
Hygromycin-resistance (HyR)-based Escherichia coli-mycobacterial shuttle plasmids have high efficiencies of transformation and a broad mycobacterial host range. We have introduced a lacZ alpha (encoding the alpha-polypeptide fragment of beta-galactosidase (beta Gal))-multiple cloning site cassette into a HyR-based shuttle vector to generate a plasmid with nine unique cloning sites and the added feature of beta Gal color selection in appropriate E. coli host strains.
基于潮霉素抗性（HyR）的大肠杆菌-分枝杆菌穿梭质粒具有高效的转化效率和广泛的分枝杆菌宿主范围。我们已将一个lacZα（编码β-半乳糖苷酶（βGal）的α-多肽片段）-多克隆位点盒引入到一个基于HyR的穿梭载体中，以产生一个具有9个独特克隆位点的质粒，并在合适的大肠杆菌宿主菌株中增加了βGal颜色筛选功能。
