将人类DNA引发酶的49 kDa（PRIM1）和58 kDa（PRIM2A和PRIM2B）亚基基因定位到染色体带1q44和6p11.1 - p12。
Assignment of the 49-kDa (PRIM1) and 58-kDa (PRIM2A and PRIM2B) subunit genes of the human DNA primase to chromosome bands 1q44 and 6p11.1-p12.
作者信息
Shiratori A, Okumura K, Nogami M, Taguchi H, Onozaki T, Inoue T, Ando T, Shibata T, Izumi M, Miyazawa H
机构信息
Division of Human Genome Research and Gene Bank, Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
出版信息
Genomics. 1995 Jul 20;28(2):350-3. doi: 10.1006/geno.1995.1155.
DNA primase is an essential replication protein that catalyzes the synthesis of oligoribonucleotide primers. DNA primase, consisting of two subunits (p49 and p58), plays a key role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis. We mapped the locations of human chromosomes of the genes coding for both subunits [p49 (PRIM1) and p58 (PRIM2)] by PCR amplification using DNAs of a panel of somatic hybrids, to chromosomes 1 and 6, respectively. The PRIM1 gene was mapped to 1q44, and two PRIM2 loci (PRIM2A and PRIM2B) were detected at 6p11.1-p12 by fluorescence in situ hybridization using several genomic DNA probes.
DNA引发酶是一种重要的复制蛋白，可催化寡核糖核苷酸引物的合成。由两个亚基（p49和p58）组成的DNA引发酶在DNA复制起始以及滞后链合成的冈崎片段合成过程中均发挥关键作用。我们利用一组体细胞杂种的DNA，通过PCR扩增将编码这两个亚基[p49（PRIM1）和p58（PRIM2）]的基因分别定位到人类染色体1和6上。PRIM1基因定位于1q44，通过使用几种基因组DNA探针进行荧光原位杂交，在6p11.1-p12处检测到两个PRIM2基因座（PRIM2A和PRIM2B）。
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