Sowden J, Morrison K, Schofield J, Putt W, Edwards Y
MRC Human Biochemical Genetics Unit, Galton Laboratory (UCL), London, United Kingdom.
Genomics. 1995 Sep 1;29(1):145-51. doi: 10.1006/geno.1995.1225.
Few of the auxiliary factors that assist RNA polymerase II in the process of mRNA chain elongation have been identified. We have isolated a novel cDNA, Tceb1l, from mouse and human sources that encodes a 163-amino-acid protein and shows a significant level of identity with a recently identified RNA polymerase II transcription elongation factor, p15. Tceb1l is highly conserved throughout vertebrates and maps to mouse chromosome 11 and to the syntenic region of human chromosome 5q31. Tceb1l shows a restricted pattern of expression in the early mouse embryo, where it is absent from the neurectoderm; later Tceb1l is expressed in the caudal region of the neural tube, followed by widespread expression in many tissues, including the brain and spinal cord. These observations are consistent with Tceb1l being an RNA polymerase II elongation factor and suggest that Tceb1l/p15-like peptides may be a new family of proteins that influence RNA elongation.
在mRNA链延伸过程中协助RNA聚合酶II的辅助因子，目前已鉴定出的寥寥无几。我们从小鼠和人类来源中分离出一种新的cDNA，即Tceb1l，它编码一种163个氨基酸的蛋白质，与最近鉴定出的RNA聚合酶II转录延伸因子p15具有显著的同源性。Tceb1l在整个脊椎动物中高度保守，定位于小鼠的11号染色体以及人类5号染色体q31的同线区域。Tceb1l在小鼠早期胚胎中呈现出受限的表达模式，神经外胚层中没有表达；之后Tceb1l在神经管的尾部区域表达，随后在包括脑和脊髓在内的许多组织中广泛表达。这些观察结果与Tceb1l作为一种RNA聚合酶II延伸因子相一致，并表明Tceb1l/p15样肽可能是影响RNA延伸的一类新的蛋白质家族。
