Chu T W, Capossela A, Coleman R, Goei V L, Nallur G, Gruen J R
Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06520-8064, USA.
Genomics. 1995 Sep 1;29(1):229-39. doi: 10.1006/geno.1995.1236.
The human major histocompatability complex contains genes of both immune and nonimmune importance. Recently, several genes encoding novel, non-HLA products have been described in this area. We have performed positional cloning of short fragment cDNA sequences from the class I region of the human MHC using a hybridization selection approach. This report describes isolation of full-length cDNA clones and partial genomic clones that encode a protein that contains two domains rich in cysteine and histidine similar to those characteristic of metal-dependent DNA binding proteins (C3HC4). The predicted protein also contains a domain thought to form a coiled-coil that may promote dimerization. A third feature is a polyglutamic acid region near the carboxyl terminus of the conceptual protein. Because of these properties, we have named this gene product acid finger protein (AFP). Although the biological role of AFP is unknown at present, one potential function is binding of nucleic acids. The gene (ZNF173) is expressed in multiple tissues and is conserved among mammals. In particular, the mouse and human coding regions are highly conserved. In addition to AFP, other related sequences have been localized to the MHC, suggesting that multiple AFP-like genes exist in this area.
人类主要组织相容性复合体包含具有免疫和非免疫重要性的基因。最近，在该区域已描述了几个编码新型非HLA产物的基因。我们使用杂交筛选方法对来自人类MHC I类区域的短片段cDNA序列进行了定位克隆。本报告描述了全长cDNA克隆和部分基因组克隆的分离，这些克隆编码一种蛋白质，该蛋白质包含两个富含半胱氨酸和组氨酸的结构域，类似于金属依赖性DNA结合蛋白（C3HC4）的特征结构域。预测的蛋白质还包含一个被认为形成卷曲螺旋的结构域，该结构域可能促进二聚化。第三个特征是在概念蛋白质的羧基末端附近有一个聚谷氨酸区域。由于这些特性，我们将该基因产物命名为酸性指蛋白（AFP）。尽管目前AFP的生物学作用尚不清楚，但一个潜在功能是与核酸结合。该基因（ZNF173）在多种组织中表达，并且在哺乳动物中保守。特别是，小鼠和人类的编码区域高度保守。除了AFP之外，其他相关序列已定位到MHC，这表明该区域存在多个AFP样基因。
