Bresler S E, Katushkina N V, Kolikov V M, Potokin J L, Vinogradskaya G N
J Chromatogr. 1977 Jan 11;130:275-80. doi: 10.1016/s0021-9673(00)89805-2.
The adsorption isotherm of influenza viruses on wide-pore glass was measured and was found to conform to Langmuir's equation. The affinity constants were estimated at three temperatures and the free energy, enthalpy and entropy of adsorption were calculated. A detailed study showed that the transition from adsorption to total elution is very sharp and proceeds under critical conditions of pH near the pK of the SiOH groups. Therefore, conditions are very favourable for the elution chromatography of viruses. An efficient procedure for the purification and simultaneous concentration of influenza viruses was developed.
测定了流感病毒在大孔玻璃上的吸附等温线，发现其符合朗缪尔方程。在三个温度下估算了亲和常数，并计算了吸附的自由能、焓和熵。详细研究表明，从吸附到完全洗脱的转变非常迅速，且在接近SiOH基团pK的pH临界条件下进行。因此，这些条件非常有利于病毒的洗脱色谱分离。开发了一种高效的流感病毒纯化和同时浓缩方法。
