皮炎芽生菌酵母裂解物抗原的等电聚焦组分用于检测犬芽生菌病的敏感性和特异性。

Sensitivity and specificity of an isoelectric focusing fraction of Blastomyces dermatitidis yeast lysate antigen for the detection of canine blastomycosis.

作者信息

Fisher M A, Bono J L, Abuodeh R O, Legendre A M, Scalarone G M

机构信息

Department of Biological Sciences, Idaho State University, Pocatello, USA.

出版信息

Mycoses. 1995 May-Jun;38(5-6):177-82. doi: 10.1111/j.1439-0507.1995.tb00046.x.

DOI:10.1111/j.1439-0507.1995.tb00046.x

PMID:8531928

Abstract

Blastomyces dermatitidis yeast lysate antigen (T-58, dog isolate) fractions prepared using the Rotofor preparative isoelectric focusing (IEF) cell (Bio-Rad) were compared with B. dermatitidis yeast lysate and filtrate reagents with respect to the detection of antibodies in sera from dogs with blastomycosis, histoplasmosis, coccidioidomycosis, cryptococcosis and aspergillosis. A horseradish peroxidase enzyme immunoassay with Turbo TMB substrate was used in the study. One particular IEF fraction (pH 4.3) was optimal in the assay, and it exhibited greater sensitivity (100%) and specificity (93%) than the lysate or filtrate preparations. The highest degree of cross-reactivity was encountered with the histoplasmosis and coccidioidomycosis specimens and considerably less with the cryptococcosis and aspergillosis sera. Studies are in progress to purify further the optimal IEF fraction.

摘要

使用Rotofor制备性等电聚焦（IEF）细胞（伯乐公司）制备的皮炎芽生菌酵母裂解物抗原（T - 58，犬分离株）组分，在检测患有芽生菌病、组织胞浆菌病、球孢子菌病、隐球菌病和曲霉病的犬血清中的抗体方面，与皮炎芽生菌酵母裂解物和滤液试剂进行了比较。该研究采用了以Turbo TMB底物的辣根过氧化物酶酶免疫测定法。一种特定的IEF组分（pH 4.3）在该测定中表现最佳，并且它比裂解物或滤液制剂具有更高的灵敏度（100%）和特异性（93%）。在组织胞浆菌病和球孢子菌病标本中遇到的交叉反应程度最高，而在隐球菌病和曲霉病血清中的交叉反应则少得多。目前正在进行进一步纯化最佳IEF组分的研究。