Boyd Alex R, Vandyke Jamie L, Scalarone Gene M
Department of Biological Sciences, Idaho State University, P.O. Box 8007 921 S. 8th Avenue, Pocatello, ID 83209, USA.
Vet Med Int. 2013;2013:940126. doi: 10.1155/2013/940126. Epub 2013 Oct 8.
The systemic fungal infection, blastomycosis, which infects both humans and animals has presented a diagnostic challenge for clinicians for many years. The aim of this study was to evaluate the diagnostic sensitivity of Blastomyces dermatitidis yeast lysate antigens with respect to antibody detection in dogs with blastomycosis. Lysate antigens were prepared from B. dermatitidis isolates T-58 and T-66 (dogs, Tennessee) and WI-R and WI-J (dogs, Wisconsin). Based on results obtained from a preliminary comparative study, five combinations of these isolates and one individual isolate were tested against 92 serum specimens from dogs with culture-proven or histologically-confirmed blastomycosis, using the indirect enzyme-linked immunosorbent assay (ELISA). Mean absorbance values obtained from the sera ranged from 0.905 with the individual T-58 antigen to 1.760 using an antigen combination (T-58 + T-66 + WI-R). All of the 6 antigenic preparations were able to detect antibody in the serum specimens, but the antigen combinations detected antibody to a higher degree than the individual antigen. This study provides evidence that combinations of the yeast lysate reagents seem to be more efficacious for antibody detection in dog sera, but our laboratory is continuing to evaluate antigen lysate combinations for detection of antibodies in blastomycosis.
系统性真菌感染——芽生菌病，可感染人类和动物，多年来一直给临床医生带来诊断挑战。本研究的目的是评估皮炎芽生菌酵母裂解物抗原对患有芽生菌病犬只抗体检测的诊断敏感性。裂解物抗原由皮炎芽生菌分离株T - 58和T - 66（来自田纳西州的犬只）以及WI - R和WI - J（来自威斯康星州的犬只）制备而成。基于初步比较研究的结果，使用间接酶联免疫吸附测定（ELISA），将这些分离株的五种组合以及一种单独的分离株与92份来自经培养证实或组织学确诊为芽生菌病犬只的血清标本进行检测。从血清中获得的平均吸光度值范围为：单独使用T - 58抗原时为0.905，使用抗原组合（T - 58 + T - 66 + WI - R）时为1.760。所有6种抗原制剂均能在血清标本中检测到抗体，但抗原组合检测抗体的程度高于单独抗原。本研究提供了证据，表明酵母裂解物试剂组合似乎对犬血清中的抗体检测更有效，但我们实验室仍在继续评估抗原裂解物组合用于芽生菌病抗体检测的情况。
