SantaLucia J, Shen L X, Cai Z, Lewis H, Tinoco I
Department of Chemistry, Lawrence Berkeley Laboratory, University of California, Berkeley 94720, USA.
Nucleic Acids Res. 1995 Dec 11;23(23):4913-21. doi: 10.1093/nar/23.23.4913.
Efficient syntheses of pyrimidine and purine nucleosides and nucleotides with selective 13C enrichment in the base moieties are described. Uridine and cytidine are labeled at position C6 and adenosine and guanosine are labeled at position C8. The selectively labeled nucleosides were converted to nucleoside triphosphates and used with in vitro transcription to synthesize labeled RNA. Isotope-edited 12C and 13C sub-spectra of a omega 1-1/2-X-filtered NOESY experiment are demonstrated to be useful for making resonance assignments and for deriving structural information in large (> 20 nt) RNA molecules. The labeled RNAs also allow heteronuclear J-couplings and relaxation parameters to be measured without complications from 13C-13C J-couplings.
描述了在碱基部分具有选择性13C富集的嘧啶和嘌呤核苷及核苷酸的高效合成方法。尿苷和胞苷在C6位标记，腺苷和鸟苷在C8位标记。将选择性标记的核苷转化为核苷三磷酸，并用于体外转录以合成标记的RNA。ω1-1/2-X滤波NOESY实验的同位素编辑12C和13C子光谱被证明可用于共振归属以及推导大（>20 nt）RNA分子的结构信息。标记的RNA还允许测量异核J耦合和弛豫参数，而不会受到13C-13C J耦合的干扰。
