van Oirschot J T, Rijsewijk F A, Straver P J, Ruuls R C, Quak J, Davidse A, Westenbrink F, Gielkens A L, van Dijk J E, Moerman A
Institute for Animal Science and Health, Lelystad, The Netherlands.
Vet Rec. 1995 Sep 2;137(10):235-9. doi: 10.1136/vr.137.10.235.
A bovine herpesvirus 1 (BHV-1) isolate from the semen of a subclinically infected bull was administered to cattle by various routes to assess its virulence. Cattle that were artificially inseminated or inoculated intrapreputially did not develop clinical signs, but did transmit the virus to contact cattle. However, the isolate induced severe signs of rhinotracheitis and vulvovaginitis in cattle that were inoculated by the intravaginal, intranasal or intravenous routes, but did not infect the fetus. The isolate was therefore not of low virulence. Analysis with DNA restriction enzymes could not assign the isolate to either the BHV-1.1 or BHV-1.2 genotype.
从一头亚临床感染公牛精液中分离出的牛疱疹病毒1型（BHV-1），通过多种途径接种给牛，以评估其毒力。经人工授精或包皮内接种的牛未出现临床症状，但将病毒传播给了接触的牛。然而，该分离株通过阴道内、鼻内或静脉内途径接种牛时，会引发严重的鼻气管炎和外阴阴道炎症状，但不会感染胎儿。因此，该分离株并非低毒力毒株。用DNA限制性内切酶分析无法将该分离株归为BHV-1.1或BHV-1.2基因型。
