[多巴和氧气对培养的鸡胚视网膜色素上皮细胞中RNA浓度的影响]

[The effects of dopa and oxygen on RNA concentrations in cultured chick embryonal retinal pigment epithelial cells].

作者信息

Kanda T, Akeo K, Murakami A, Karasawa Y, Okisaka S

机构信息

Department of Ophthalmology, National Defense Medical College, Saitama-ken, Japan.

出版信息

Nippon Ganka Gakkai Zasshi. 1995 Oct;99(10):1123-6.

PMID:8533633

Abstract

We measured RNA and DNA concentrations in cultured chick embryonal retinal pigment epithelial cells to investigate the effects of dopa and oxygen on DNA and RNA synthesis. RNA/DNA ratios were decreased by addition of 250 microM dopa. Decrease of RNA/DNA ratios was suppressed when the oxygen concentrations were reduced from 20% to 10%. Incubation with medium containing 100 microM dopa increased RNA/DNA ratios in 10% oxygen. Exposure of retinal pigment epithelial cells to 250 microM dopa caused the decrease of RNA concentrations in the retinal pigment epithelial cells, which was ameliorated by lowering oxygen concentrations. However, the addition of 100 microM dopa in 10% oxygen stimulated retinal pigment epithelial cells and seemed to increase RNA concentrations.

摘要

我们测量了培养的鸡胚视网膜色素上皮细胞中的RNA和DNA浓度，以研究多巴和氧气对DNA和RNA合成的影响。添加250微摩尔多巴会降低RNA/DNA比率。当氧气浓度从20%降至10%时，RNA/DNA比率的降低受到抑制。在含100微摩尔多巴的培养基中孵育会使在10%氧气环境下的RNA/DNA比率升高。视网膜色素上皮细胞暴露于250微摩尔多巴会导致视网膜色素上皮细胞中RNA浓度降低，降低氧气浓度可改善这一情况。然而，在10%氧气环境中添加100微摩尔多巴会刺激视网膜色素上皮细胞，似乎会增加RNA浓度。