Muraki Y, Akeo K, Tanaka Y, Fujiwara T
Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
Nippon Ganka Gakkai Zasshi. 1995 Nov;99(11):1203-8.
In order to make clear how the ultrastructure of the bovine second-passage retinal pigment epithelium (RPE) was affected by exposure to L-dopa and oxygen, we compared the toxic effects of melanotic and amelanotic RPE. The oxygen levels in the incubators and the L-dopa concentrations in the media were adjusted to 10% or 20% oxygen by adding nitrogen gas and 100 microM or 250 microM L-dopa, respectively. After 24 hours, melanotic and amelanotic RPE were fixed and embedded in epon. Ultrathin sections were examined by transmission electron microscopy. Rough endoplasmic reticula were found to be dilated and ribosomes were extinguished in both melanotic and amelanotic RPE. The damage by L-dopa in melanotic RPE was less than that in amelanotic RPE and less in 10% oxygen than in 20% oxygen.
为了弄清楚牛第二代视网膜色素上皮（RPE）的超微结构如何受到L-多巴和氧气暴露的影响，我们比较了有黑色素的和无黑色素的RPE的毒性作用。通过分别添加氮气将培养箱中的氧气水平和培养基中的L-多巴浓度分别调整为10%或20%的氧气以及100微摩尔或250微摩尔的L-多巴。24小时后，将有黑色素的和无黑色素的RPE固定并包埋在环氧树脂中。通过透射电子显微镜检查超薄切片。发现有黑色素的和无黑色素的RPE中的粗面内质网均扩张且核糖体消失。L-多巴对有黑色素的RPE的损伤小于对无黑色素的RPE的损伤，且在10%氧气环境中的损伤小于在20%氧气环境中的损伤。
