Béraud-Colomb E, Roubin R, Martin J, Maroc N, Gardeisen A, Trabuchet G, Goosséns M
INSERM U406, Génétique Médicale et Développement, Marseille, France.
Am J Hum Genet. 1995 Dec;57(6):1267-74.
Analyzing the nuclear DNA from ancient human bones is an essential step to the understanding of genetic diversity in current populations, provided that such systematic studies are experimentally feasible. This article reports the successful extraction and amplification of nuclear DNA from the beta-globin region from 5 of 10 bone specimens up to 12,000 years old. These have been typed for beta-globin frameworks by sequencing through two variable positions and for a polymorphic (AT) chi (T) gamma microsatellite 500 bp upstream of the beta-globin gene. These specimens of human remains are somewhat older than those analyzed in previous nuclear gene sequencing reports and considerably older than those used to study high-copy-number human mtDNA. These results show that the systematic study of nuclear DNA polymorphisms of ancient populations is feasible.
倘若此类系统性研究在实验上可行，那么分析古代人类骨骼中的核DNA是理解当前人群遗传多样性的关键一步。本文报告了从10个骨标本中的5个成功提取并扩增出高达12000年历史的β-珠蛋白区域的核DNA。通过对两个可变位置进行测序以及对β-珠蛋白基因上游500 bp处的多态性（AT）χ（T）γ微卫星进行分析，确定了这些样本的β-珠蛋白框架类型。这些人类遗骸样本比之前核基因测序报告中分析的样本年代稍久，比用于研究高拷贝数人类线粒体DNA的样本年代久远得多。这些结果表明，对古代人群核DNA多态性进行系统性研究是可行的。
