来自苏云金芽孢杆菌jegathesan亚种的一个编码高杀蚊蛋白的新型毒素基因的克隆与表达。
Cloning and expression of a novel toxin gene from Bacillus thuringiensis subsp. jegathesan encoding a highly mosquitocidal protein.
作者信息
Delécluse A, Rosso M L, Ragni A
机构信息
Unité des Bactéries Entomopathogènes, Institut Pasteur, Paris, France.
出版信息
Appl Environ Microbiol. 1995 Dec;61(12):4230-5. doi: 10.1128/aem.61.12.4230-4235.1995.
Abstract
A gene, designated cry11B, encoding a 81,293-Da crystal protein of Bacillus thuringiensis subsp. jegathesan was cloned by using a gene-specific oligonucleotide probe. The sequence of the Cry11B protein, as deduced from the sequence of the cry11B gene, contains large regions of similarity with the Cry11A toxin (previously CryIVD) from B. thuringiensis subsp. israelensis. The Cry11B protein was immunologically related to both Cry11A and Cry4A proteins. The cry11B gene was expressed in a nontoxic strain of B. thuringiensis, in which Cry11B was produced in large amounts during sporulation and accumulated as inclusions. Purified Cry11B inclusions were highly toxic for mosquito larvae of the species Aedes aegypti, Culex pipiens, and Anopheles stephensi. The activity of Cry11B toxin was higher than that of Cry11A and similar to that of the native crystals from B. thuringiensis subsp. jegathesan, which contain at least seven polypeptides.
摘要
利用基因特异性寡核苷酸探针克隆了一个名为cry11B的基因，该基因编码苏云金芽孢杆菌jegathesan亚种的一种81293道尔顿的晶体蛋白。从cry11B基因序列推导的Cry11B蛋白序列，与苏云金芽孢杆菌以色列亚种的Cry11A毒素（以前的CryIVD）有大片段的相似性。Cry11B蛋白在免疫上与Cry11A和Cry4A蛋白相关。cry11B基因在苏云金芽孢杆菌的一个无毒菌株中表达，在该菌株中，Cry11B在芽孢形成过程中大量产生并以包涵体形式积累。纯化的Cry11B包涵体对埃及伊蚊、致倦库蚊和斯氏按蚊的幼虫具有高毒性。Cry11B毒素的活性高于Cry11A，与苏云金芽孢杆菌jegathesan亚种的天然晶体相似，后者至少含有七种多肽。
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