Nicolas L, Nielsen-Leroux C, Charles J F, Delécluse A
Unité des Bactéries Entomopathogènes, Institut Pasteur, Paris, France.
FEMS Microbiol Lett. 1993 Feb 1;106(3):275-80. doi: 10.1111/j.1574-6968.1993.tb05976.x.
The 42- and 51-kDa protein genes of Bacillus sphaericus 1593 have been subcloned independently downstream from the cytA gene promoter of Bacillus thuringiensis serovar israelensis and introduced into a non-mosquitocidal strain of Bacillus thuringiensis. Consequently, each protein was overproduced and accumulated as inclusion bodies which were purified. For the first time, the 42-kDa protein inclusions alone were found to be toxic to Culex pipiens larvae (LC50 at 48 h 300 ng ml-1); in contrast, the 51-kDa protein inclusions were not. Moreover, a synergistic effect between these two components was observed.
球形芽孢杆菌1593的42 kDa和51 kDa蛋白基因已分别亚克隆到苏云金芽孢杆菌以色列亚种cytA基因启动子的下游，并导入苏云金芽孢杆菌的一个非杀蚊菌株中。因此，每种蛋白都过量产生并以包涵体形式积累，随后对包涵体进行了纯化。首次发现仅42 kDa蛋白包涵体对致倦库蚊幼虫有毒性（48小时的LC50为300 ng/ml）；相比之下，51 kDa蛋白包涵体则没有毒性。此外，还观察到这两种成分之间存在协同效应。
