谷氨酸棒杆菌trpD基因中的突变通过编码一种抗反馈的邻氨基苯甲酸磷酸核糖基转移酶赋予对5-甲基色氨酸的抗性。
Mutations in the trpD gene of Corynebacterium glutamicum confer 5-methyltryptophan resistance by encoding a feedback-resistant anthranilate phosphoribosyltransferase.
作者信息
O'Gara J P, Dunican L K
机构信息
Department of Microbiology, University College Galway, Ireland.
出版信息
Appl Environ Microbiol. 1995 Dec;61(12):4477-9. doi: 10.1128/aem.61.12.4477-4479.1995.
Abstract
The trpD gene from tryptophan-hyperproducing Corynebacterium glutamicum ATCC 21850 was isolated on the basis of its ability to confer resistance to 5-methyltryptophan on wild-type C. glutamicum AS019. Comparative sequence analysis of the genes from the wild-type AS019 and ATCC 21850 trpD genes revealed two amino acid substitutions at the protein level. Further analysis demonstrated that the trpD gene product from ATCC 21850, anthranilate phosphoribosyltransferase, was more resistant to feedback inhibition by either tryptophan or 5-methyltryptophan than its wild-type counterpart. It is proposed that phosphoribosyltransferase insensitivity to tryptophan in ATCC 21850 contributes to an elevated level of tryptophan biosynthesis.
摘要
基于其赋予野生型谷氨酸棒杆菌AS019对5-甲基色氨酸抗性的能力，从色氨酸高产谷氨酸棒杆菌ATCC 21850中分离出trpD基因。对野生型AS019和ATCC 21850的trpD基因进行比较序列分析，结果显示在蛋白质水平上有两个氨基酸替换。进一步分析表明，来自ATCC 21850的trpD基因产物邻氨基苯甲酸磷酸核糖基转移酶比其野生型对应物对色氨酸或5-甲基色氨酸的反馈抑制更具抗性。有人提出，ATCC 21850中磷酸核糖基转移酶对色氨酸不敏感有助于提高色氨酸生物合成水平。
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