后期染色单体运动：II型DNA拓扑异构酶的作用
Anaphase chromatid motion: involvement of type II DNA topoisomerases.
作者信息
Duplantier B, Jannink G, Sikorav J L
机构信息
Service de Physique Théorique, CEA/Saclay, Gif-sur-Yvette, France.
出版信息
Biophys J. 1995 Oct;69(4):1596-605. doi: 10.1016/S0006-3495(95)80032-9.
Abstract
Sister chromatids are topologically intertwined at the onset of anaphase: their segregation during anaphase is known to require strand-passing activity by type II DNA topoisomerase. We propose that the removal of the intertwinings involves at the same time the traction of the mitotic spindle and the activity of topoisomerases. This implies that the velocity of the chromatids is compatible with the kinetic constraints imposed by the enzymatic reaction. We show that the greatest observed velocities (about 0.1 microns s-1) are close to the theoretical upper bound compatible with both the diffusion rate (calculated here within a probabilistic model) and the measured reaction rate of the enzyme.
摘要
在后期开始时，姐妹染色单体在拓扑结构上相互缠绕：已知它们在后期的分离需要II型DNA拓扑异构酶的链通过活性。我们提出，去除缠绕同时涉及有丝分裂纺锤体的牵引和拓扑异构酶的活性。这意味着染色单体的速度与酶促反应所施加的动力学限制是相容的。我们表明，观察到的最大速度（约0.1微米/秒）接近与扩散速率（在此概率模型内计算）和酶的测量反应速率都相容的理论上限。
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本文引用的文献
1
On the coupling between ATP usage and DNA transport by yeast DNA topoisomerase II.酵母DNA拓扑异构酶II介导的ATP利用与DNA转运之间的偶联
J Biol Chem. 1993 Apr 15;268(11):8096-104.
2
Multiple chromosomal populations of topoisomerase II detected in vivo by time-lapse, three-dimensional wide-field microscopy.通过延时三维宽视场显微镜在体内检测到的拓扑异构酶II的多个染色体群体。
Cell. 1993 Apr 9;73(1):97-108. doi: 10.1016/0092-8674(93)90163-k.
3
Nature and distribution of chromosomal intertwinings in Saccharomyces cerevisiae.酿酒酵母中染色体缠绕的性质与分布
Mol Cell Biol. 1994 Feb;14(2):1465-76. doi: 10.1128/mcb.14.2.1465-1476.1994.
4
Chromosomal replication initiates and terminates at random sequences but at regular intervals in the ribosomal DNA of Xenopus early embryos.
EMBO J. 1993 Dec;12(12):4511-20. doi: 10.1002/j.1460-2075.1993.tb06140.x.
5
Inverse relationship between catenation and superhelicity in newly replicated simian virus 40 daughter chromosomes.新复制的猴病毒40子代染色体中连环化与超螺旋性之间的负相关关系。
Biochem Biophys Res Commun. 1994 Jun 30;201(3):1510-7. doi: 10.1006/bbrc.1994.1875.
6
Coming undone: how to untangle a chromosome.解开谜团：如何解开染色体之谜。
Cell. 1994 Jul 1;77(7):955-7. doi: 10.1016/0092-8674(94)90433-2.
7
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Cell. 1995 Apr 21;81(2):253-60. doi: 10.1016/0092-8674(95)90335-6.
8
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Annu Rev Biophys Biomol Struct. 1993;22:27-65. doi: 10.1146/annurev.bb.22.060193.000331.
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10
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