Yamamoto M M, Mori Y, Osada K, Murakami H
National Research Institute of Vegetables, Shizuoka, Japan.
Biosci Biotechnol Biochem. 1995 Oct;59(10):1842-5. doi: 10.1271/bbb.59.1842.
The promotive effects of poly-cations on immunoglobulin production was investigated using human-human hybridoma cells. Among poly-cations tested, epsilon-poly-L-lysine with hydrochloride (approximately 4 kDa), which has been used as an antibacterial food additive, had the greatest activity in enhancing IgM production of human-human hybridoma HB4C5 cells without stimulating cell proliferation. Immunoglobulin production stimulatory (IPS) activity of epsilon-poly-lysine was not affected by trypsin digestion. It was stable below 60 degrees C but completely inactivated with heating at 100 degrees C for 30 min. epsilon-Poly-lysine also enhanced interferon-beta (IFN-beta) production of human osteosarcoma MG-63 cells, but this stimulatory effect was reduced by the trypsin digestion.
使用人-人杂交瘤细胞研究了聚阳离子对免疫球蛋白产生的促进作用。在所测试的聚阳离子中，已用作抗菌食品添加剂的盐酸ε-聚-L-赖氨酸（约4 kDa）在增强人-人杂交瘤HB4C5细胞的IgM产生方面具有最大活性，且不刺激细胞增殖。ε-聚赖氨酸的免疫球蛋白产生刺激（IPS）活性不受胰蛋白酶消化的影响。它在60摄氏度以下稳定，但在100摄氏度加热30分钟会完全失活。ε-聚赖氨酸还增强了人骨肉瘤MG-63细胞的干扰素-β（IFN-β）产生，但这种刺激作用会因胰蛋白酶消化而降低。
