Yoshioka H, Nonaka K, Fukuda K, Kazama S
Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Japan.
Biosci Biotechnol Biochem. 1995 Oct;59(10):1901-4. doi: 10.1271/bbb.59.1901.
Chitosan derivatives, sulfated N-acyl-chitosan (S-Cn-chitosan) possessing various lengths of alkyl chain, were prepared, and the properties of their aqueous solutions were examined. The 1H-NMR spectrum of D2O solutions of S-C12-chitosan showed broadening of the proton signals caused by aggregation of the alkyl chain. The solubility of a hydrophobic compounds, azobenzene, was small in the aqueous solutions of S-Cn-chitosan with shorter alkyl chains, but increased with increasing length of the chains above C10, showing that micelles had been formed. The ESR spectrum of a spin probe, TEMPO, in an S-C14-chitosan solution showed the existence of a hydrophobic region in the solution, but this region did not exist in the S-C2-chitosan solution. The rigidity of this region was examined by using a spin probe, 16-doxyl-stearic acid. From these results, it was revealed that S-Cn-chitosan with longer alkyl chains formed a novel type of micelle called a "polymer micelle," which was more stable than the ordinary micelles formed from low-molecular-weight surfactants.
制备了具有不同烷基链长度的壳聚糖衍生物——硫酸化N-酰基壳聚糖（S-Cn-壳聚糖），并研究了其水溶液的性质。S-C12-壳聚糖的D2O溶液的1H-NMR谱显示，由于烷基链的聚集，质子信号变宽。在具有较短烷基链的S-Cn-壳聚糖水溶液中，疏水性化合物偶氮苯的溶解度较小，但当链长超过C10时，其溶解度随链长增加而增大，表明形成了胶束。自旋探针TEMPO在S-C14-壳聚糖溶液中的ESR谱显示溶液中存在疏水区域，但在S-C2-壳聚糖溶液中不存在该区域。使用自旋探针16-脱氧硬脂酸研究了该区域的刚性。从这些结果可以看出，具有较长烷基链的S-Cn-壳聚糖形成了一种新型胶束，称为“聚合物胶束”，它比由低分子量表面活性剂形成的普通胶束更稳定。
