Ianăş O, Olinescu R, Bădescu I, Simionescu L, Popovici D
C. I. Parhon Institute of Endocrinology, Bucharest, Romania.
Rom J Intern Med. 1995 Jan-Jun;33(1-2):113-20.
The hepatoprotective action of the Romanian preparation Orgasel containing selenium (Se) 5.01 mg/100 g autolysated yeast powder, was tested on adult Wistar rats poisoned with carbon tetrachloride (CCl4). The hepatotoxic agent (a 20% CCl4 solution in oil) was administered i.p. in a single dose of 0.3 ml CCl4 solution/100 g body weight, and the preparation tested (autolysate of seleniated yeast) was administered by gavage in 4 doses (of 100 mg Se powder/100 g animal each) along 2 days. After 48 hrs the animals were sacrificed, then their blood and liver were collected. The treatment with Orgasel significantly reduced the organs, morphological changes (fat liver degeneration, splenomegaly, testicle degeneration) induced by CCl4 poisoning in the rat, an effect found also at the biological parameters levels studied in plasma and liver. In the plasma, the high lipid peroxide concentrations, the increased activity of alkaline phosphatases, and the reduced antioxidative activity generated by CCl4 have been statistically significant brought to the normal range after Orgasel administration. At the liver this treatment significantly decreased the lipid peroxides production, the total lipids and cholesterol concentrations, and statistically significant increased the enzymes activity (alkaline phosphatases, GPT). The results obtained after Orgasel administration proved that this preparation has a global beneficial action upon the organism in the poisoned rat, as well as a strong antioxidative effect, confirming once again the essential role of Se in maintaining cells' integrity.
对成年Wistar大鼠进行实验，以测试罗马尼亚制剂奥尔加塞尔（Orgasel）的肝脏保护作用。该制剂含有5.01毫克/100克自溶酵母粉形式的硒（Se），实验对象是用四氯化碳（CCl4）中毒的成年Wistar大鼠。肝毒性剂（20%的CCl4油溶液）经腹腔注射，单剂量为0.3毫升CCl4溶液/100克体重，而受试制剂（硒化酵母自溶物）通过灌胃分4次给药（每次100毫克硒粉/100克动物体重），持续2天。48小时后处死动物，然后采集血液和肝脏。用奥尔加塞尔治疗可显著减轻大鼠因CCl4中毒引起的器官形态变化（脂肪肝变性、脾肿大、睾丸变性），在血浆和肝脏中所研究的生物学参数水平上也发现了这种效果。在血浆中，CCl4导致的高脂质过氧化物浓度、碱性磷酸酶活性增加以及抗氧化活性降低，在给予奥尔加塞尔后经统计学分析已显著恢复至正常范围。在肝脏方面，这种治疗显著降低了脂质过氧化物的产生、总脂质和胆固醇浓度，并在统计学上显著提高了酶活性（碱性磷酸酶、谷丙转氨酶）。给予奥尔加塞尔后获得的结果证明，该制剂对中毒大鼠的机体具有全面的有益作用以及强大的抗氧化作用，再次证实了硒在维持细胞完整性方面的重要作用。
