Migot F, Chougnet C, Henzel D, Dubois B, Jambou R, Fievet N, Deloron P
INSERM U13/Institut de Médecine et d'Epidémiologie Africaines, Paris, France.
Clin Exp Immunol. 1995 Dec;102(3):529-34. doi: 10.1111/j.1365-2249.1995.tb03848.x.
The central highlands of Madagascar offer a unique opportunity to explore the malaria immune memory, as the last murderous epidemic in the study area occurred 8 years ago. Quantification of the circulating memory B lymphocytes reacting to Plasmodium falciparum was assessed among 14 Madagascans by using a limiting dilution assay, applied to the EL4 culture system, which leads to activation, proliferation and differentiation into antibody-secreting cells (ASC) of most peripheral B cells. This system allowed us to observe, without any malaria-specific restimulation, a geometric mean frequency of one anti-P. falciparum ASC among 2992 circulating B cells, except for one Madagascan who did not have any detectable ASC. A geometric mean frequency of one anti-P. falciparum ASC among 1403 was obtained for six malaria hyperimmune Cameroonians, but conversely, no anti-malaria ASC was detected in the blood of six malaria non-immune French control subjects. Anti-P. falciparum ASC frequencies and serum specific antibodies were strongly related. Our results indicate that anti-malaria ASC are still present in peripheral blood of Madagascan subjects, who have not been exposed to P. falciparum for several years. These responder B cells reflect the malaria B cell memory acquired during the last epidemic.
马达加斯加的中央高地为探索疟疾免疫记忆提供了独特的机会，因为研究区域内上一次致命的疟疾流行发生在8年前。通过使用极限稀释分析，对14名马达加斯加人进行了评估，以量化对恶性疟原虫产生反应的循环记忆B淋巴细胞，该分析应用于EL4培养系统，该系统可使大多数外周B细胞激活、增殖并分化为抗体分泌细胞（ASC）。该系统使我们能够在没有任何疟疾特异性再刺激的情况下，观察到在2992个循环B细胞中有一个抗恶性疟原虫ASC的几何平均频率，只有一名马达加斯加人未检测到任何ASC。对于六名疟疾超免疫的喀麦隆人，在1403个细胞中有一个抗恶性疟原虫ASC的几何平均频率，但相反，在六名疟疾非免疫的法国对照受试者的血液中未检测到抗疟疾ASC。抗恶性疟原虫ASC频率与血清特异性抗体密切相关。我们的结果表明，在多年未接触恶性疟原虫的马达加斯加受试者的外周血中仍存在抗疟疾ASC。这些反应性B细胞反映了在上一次疟疾流行期间获得的疟疾B细胞记忆。
