Hamilton J L, Pollard Y, Grant D, Patterson K, Machin S J
Department of Haematology, University College Hospital, London, UK.
Clin Lab Haematol. 1995 Jun;17(2):145-9.
Reticulocyte counting was assessed on the Coulter STKS-2A automated blood cell counter. Using a two step procedure, blood samples were first incubated with the supravital stain new methylene blue. An acidic reagent was then added to clear the haemoglobin and any stained RNA was preserved within the cell. The cells were then analysed by measurement of volume, conductivity and light scatter (VCS). The results of 123 samples analysed on the STKS-2A were compared with those from a Toa Sysmex R-1000 reticulocyte counter. One hundred and seven samples gave no review flags and reticulocyte counts ranging from 0.5% to 22.8%, resulting in a correlation coefficient of 0.93 for the methods. Between run imprecision studies gave CVs ranging from 5.3% for a reticulocyte count of 8.7% to a CV of 16.3% for a 0.34% count. Stability studies showed insignificant changes over 72 h storage. These findings confirm that VCS technology can be adapted to provide precise and accurate routine reticulocyte analysis.
使用库尔特STKS - 2A全自动血细胞计数器评估网织红细胞计数。采用两步法，首先将血样与超活染色剂新亚甲蓝孵育。然后加入酸性试剂清除血红蛋白，细胞内任何染色的RNA得以保留。接着通过测量体积、电导率和光散射（VCS）对细胞进行分析。将在STKS - 2A上分析的123份样本的结果与来自东亚Sysmex R - 1000网织红细胞计数器的结果进行比较。107份样本无审核标记，网织红细胞计数范围为0.5%至22.8%，两种方法的相关系数为0.93。批间不精密度研究得出，网织红细胞计数为8.7%时变异系数为5.3%，计数为0.34%时变异系数为16.3%。稳定性研究表明，储存72小时内变化不显著。这些结果证实，VCS技术可用于提供精确且准确的常规网织红细胞分析。
