Tatsumi N, Tsuda I, Yokomatsu Y, Im T S, Niri M, Furota A
Osaka City Med J. 1989 Jun;35(1):39-47.
Reticulocyte count is an essential clinical test to detect erythropoietic activity. The test has been done manually with the use of one of three dyes: new methylene blue, brilliant cresyl blue, and azure B. When reticulocyte counts with the different dyes were compared, correlation was good. When reticulocyte counts obtained by use of the dyes were compared with the count obtained by an automated counter, correlation was high. In a sampling study, the specimen prepared by a spinner method gave smaller variations than a wedge method. Intertechnologist bias was higher than inter-specimen or intra-specimen bias: The variation of the reticulocyte count was examined with from 200 to 2,000 cells; with 1,000 cells or more, variation was low. From these results, we concluded that more than 1,000 erythrocytes on one slide prepared by the spinner method should be analyzed. Clear standards for cell identification are also needed.
网织红细胞计数是检测红细胞生成活性的一项重要临床检查。该检查过去是使用三种染料之一手动进行的：新亚甲蓝、煌焦油蓝和天青B。比较使用不同染料进行的网织红细胞计数时，相关性良好。将使用这些染料获得的网织红细胞计数与自动计数仪获得的计数进行比较时，相关性很高。在一项抽样研究中，通过旋转法制备的标本比楔形法产生的变异更小。技术人员之间的偏差高于标本间或标本内偏差：在检查200至2000个细胞的网织红细胞计数变异时；计数1000个或更多细胞时，变异较低。根据这些结果，我们得出结论，对于通过旋转法制备的一张载玻片上的红细胞，应分析1000个以上。还需要明确的细胞识别标准。
