胰腺癌患者十二指肠液中Ki-ras密码子12突变的分析。

Analysis of Ki-ras codon 12 mutations in the duodenal juice of patients with pancreatic cancer.

作者信息

Iguchi H, Sugano K, Fukayama N, Ohkura H, Sadamoto K, Ohkoshi K, Seo Y, Tomoda H, Funakoshi A, Wakasugi H

机构信息

Department of Biochemistry, National Kyushu Cancer Center, Fukuoka, Japan.

出版信息

Gastroenterology. 1996 Jan;110(1):221-6. doi: 10.1053/gast.1996.v110.pm8536860.

DOI:10.1053/gast.1996.v110.pm8536860

PMID:8536860

Abstract

BACKGROUND & AIMS: Point mutations of the Ki-ras gene at codon 12 have been frequently identified in pure pancreatic juice of patients with pancreatic cancer in studies examining pancreatic cancer tissues. The aim of this study was to examine mutations of the Ki-ras codon 12 in the duodenal juice collected from patients with various pancreatic disorders.

METHODS

The duodenal juice was collected through a Dreiling tube installed in the duodenum during a secretin test. Analysis of the Ki-ras mutations was performed using the enriched polymerase chain reaction--single-strand conformation polymorphism technique.

RESULTS

Point mutations were detected in 12 of 19 patients with pancreatic cancer; of the 12 patients, 10 had ductal tubular adenocarcinoma and 2 intraductal papillary adenocarcinoma. Mutational patterns included GAT (n = 4), GTT (n = 3), CGT (n = 1), and double mutations of GTT and GAT (n = 3) and GAT and CGT (n = 1). In 41 patients with benign pancreatic disorders, a point mutation was detected in only 1 patient with chronic pancreatitis.

CONCLUSIONS

Analysis of the Ki-ras codon 12 mutations in the duodenal juice is useful in the diagnosis of pancreatic cancer.

摘要

背景与目的

在胰腺癌组织研究中，经常在胰腺癌患者的纯胰液中检测到第12密码子处的Ki-ras基因突变。本研究旨在检测从患有各种胰腺疾病的患者收集的十二指肠液中Ki-ras第12密码子的突变情况。

方法

在促胰液素试验期间，通过安装在十二指肠的德赖林管收集十二指肠液。使用富集聚合酶链反应-单链构象多态性技术对Ki-ras突变进行分析。

结果

19例胰腺癌患者中有12例检测到点突变；在这12例患者中，10例为导管管状腺癌，2例为导管内乳头状腺癌。突变模式包括GAT（n = 4）、GTT（n = 3）、CGT（n = 1），以及GTT与GAT（n = 3）和GAT与CGT（n = 1）的双重突变。在41例良性胰腺疾病患者中，仅1例慢性胰腺炎患者检测到点突变。