Harada M, Seta K, Ito O, Tamada K, Li T, Terao H, Takenoyama M, Kimura G, Nomoto K
Department of Virology and Immunology, Kyushu University, Fukuoka, Japan.
Immunopharmacol Immunotoxicol. 1995 Nov;17(4):687-703. doi: 10.3109/08923979509037189.
The oral administration of a kampo herbal medicine, Hochu-ekki-to (TJ-41: Bu-Zhong-Yi-Qi-Tang) using a water-supplying bottle resulted in a slight but significant inhibition of Meth A growth. The oral administration of TJ-41 with gastric gavage significantly enhanced the specific antitumor activity against Meth A at rechallenge on day 9. In a tumor-neutralizing assay, the tumor draining LN cells of the TJ-41 administered mice showed an antitumor activity against Meth A. In a cytolytic assay, the anti-Meth A specific cytolytic T lymphocyte activity was not detected in the spleen cells of the Meth A bearing and TJ-41 administered mice. The oral administration of TJ-41 enhanced the natural killer (NK) activity of the spleen cells in naive mice but could not improve the decreased NK activity of spleen cells from the tumor bearing mice. In a cytostatic assay, the peritoneal exudate cells from the Meth A bearing and TJ-41 administered mice showed a significantly higher amount of cytostatic activity against Meth A than that from either Meth A bearing or TJ-41 administered mice. These results indicate that the oral administration of TJ-41 into the tumor bearing mice may thus be able to enhance concomitant antitumor immunity through the augmentation of the cytostatic activity.
使用供水瓶口服汉方草药补中益气汤（TJ - 41）可对Meth A肿瘤生长产生轻微但显著的抑制作用。在第9天再次接种时，通过灌胃口服TJ - 41可显著增强对Meth A的特异性抗肿瘤活性。在肿瘤中和试验中，给予TJ - 41的小鼠的肿瘤引流淋巴结细胞显示出对Meth A的抗肿瘤活性。在细胞溶解试验中，在荷Meth A肿瘤且给予TJ - 41的小鼠的脾细胞中未检测到抗Meth A特异性细胞溶解T淋巴细胞活性。口服TJ - 41可增强未接触过抗原的小鼠脾细胞的自然杀伤（NK）活性，但不能改善荷瘤小鼠脾细胞降低的NK活性。在细胞生长抑制试验中，荷Meth A肿瘤且给予TJ - 41的小鼠的腹腔渗出细胞对Meth A的细胞生长抑制活性显著高于单独荷Meth A肿瘤或单独给予TJ - 41的小鼠。这些结果表明，给荷瘤小鼠口服TJ - 41可能通过增强细胞生长抑制活性来增强伴随的抗肿瘤免疫。
