Lolova I S, Lolov S R
Institute of Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Mech Ageing Dev. 1995 Aug 8;82(2-3):73-89. doi: 10.1016/0047-6374(95)01599-u.
Immunohistochemistry and computer-assisted image analysis were used to examine the age-related changes in bFGF-immunoreactivity in rat substantia nigra (SN). Distribution pattern, number, size and staining intensity of bFGF-immunoreactive (bFGF-ir) cells in pars compacta and pars reticulata of 3-, 12- and 26-month-old rats were compared. The overall distribution of bFGF-immunoreactivity was similar in the three age groups, but changes in the morphological appearance of bFGF-ir somata and processes occurred in aging. The results demonstrated a significantly reduced number of bFGF-ir cells in pars compacta (by 56.87%) and pars reticulata (by 30.4%) in 26-month-old rats compared to 3-month-old rats. The reduction of the cell number did not occur smoothly and equally in the two parts of SN. The quantitative analysis clearly indicated a significant decrease in the size of bFGF-ir neurons in pars compacta (by 18.1%) and pars reticulata (by 14.15%) of 26-month-old rats compared to 3-month-old rats. Compared to 3-month-old rats, a 19.77% and 17.83% increase in the staining intensity was observed in the remaining bFGF-ir neurons of pars compacta and pars reticulata, respectively in 26-month-old rats. Since there was no correlation between the decreased size and increased staining, it is most probable that the intensification of the staining intensity of bFGF-ir neurons was a compensatory response to the cell death.
采用免疫组织化学和计算机辅助图像分析技术，研究大鼠黑质（SN）中碱性成纤维细胞生长因子（bFGF）免疫反应性的年龄相关变化。比较了3月龄、12月龄和26月龄大鼠致密部和网状部中bFGF免疫反应性（bFGF-ir）细胞的分布模式、数量、大小和染色强度。三个年龄组中bFGF免疫反应性的总体分布相似，但衰老过程中bFGF-ir胞体和突起的形态外观发生了变化。结果表明，与3月龄大鼠相比，26月龄大鼠致密部bFGF-ir细胞数量显著减少（减少56.87%），网状部减少30.4%。黑质这两个部分的细胞数量减少并非平稳且均匀地发生。定量分析清楚地表明，与3月龄大鼠相比，26月龄大鼠致密部bFGF-ir神经元大小显著减小（减小18.1%），网状部减小14.15%。与3月龄大鼠相比，26月龄大鼠致密部和网状部剩余bFGF-ir神经元的染色强度分别增加了19.77%和17.83%。由于细胞大小减小与染色增加之间无相关性，因此bFGF-ir神经元染色强度增强很可能是对细胞死亡的一种代偿反应。
