一种双极驱动蛋白。
A bipolar kinesin.
作者信息
Kashina A S, Baskin R J, Cole D G, Wedaman K P, Saxton W M, Scholey J M
机构信息
Section of Molecular and Cellular Biology, University of California, Davis 95616, USA.
出版信息
Nature. 1996 Jan 18;379(6562):270-2. doi: 10.1038/379270a0.
Abstract
Chromosome segregation during mitosis depends on the action of the mitotic spindle, a self-organizing, bipolar protein machine which uses microtubules (MTs) and their associated motors. Members of the BimC subfamily of kinesin-related MT-motor proteins are believed to be essential for the formation and functioning of a normal bipolar spindle. Here we report that KRP130, a homotetrameric BimC-related kinesin purified from Drosophila melanogaster embryos, has an unusual ultrastructure. It consists of four kinesin-related polypeptides assembled into a bipolar aggregate with motor domains at opposite ends, analogous to a miniature myosin filament. Such a bipolar 'minifilament' could crosslink spindle MTs and slide them relative to one another. We do not know of any other MT motors that have a bipolar structure.
摘要
有丝分裂过程中的染色体分离依赖于有丝分裂纺锤体的作用，有丝分裂纺锤体是一种自组织的双极蛋白质机器，它利用微管（MTs）及其相关的马达蛋白。驱动蛋白相关的微管马达蛋白的BimC亚家族成员被认为对于正常双极纺锤体的形成和功能至关重要。在此我们报告，KRP130是一种从黑腹果蝇胚胎中纯化出来的同四聚体BimC相关驱动蛋白，它具有不同寻常的超微结构。它由四条驱动蛋白相关多肽组装成一个双极聚集体，其马达结构域位于相对的两端，类似于一条微型肌球蛋白丝。这样一个双极“微丝”可以交联纺锤体微管并使它们相互滑动。我们不知道还有任何其他具有双极结构的微管马达蛋白。
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本文引用的文献
1
Chromosomes take the lead in spindle assembly.染色体在纺锤体组装过程中起主导作用。
Trends Cell Biol. 1995 Aug;5(8):297-301. doi: 10.1016/s0962-8924(00)89045-5.
2
ELECTRON MICROSCOPE STUDIES ON THE STRUCTURE OF NATURAL AND SYNTHETIC PROTEIN FILAMENTS FROM STRIATED MUSCLE.横纹肌天然及合成蛋白细丝结构的电子显微镜研究
J Mol Biol. 1963 Sep;7:281-308. doi: 10.1016/s0022-2836(63)80008-x.
3
Changes in the cross-striations of muscle during contraction and stretch and their structural interpretation.肌肉收缩和伸展过程中横纹的变化及其结构解释。
Nature. 1954 May 22;173(4412):973-6. doi: 10.1038/173973a0.
4
Structural changes in muscle during contraction; interference microscopy of living muscle fibres.肌肉收缩时的结构变化；活肌纤维的干涉显微镜检查
Nature. 1954 May 22;173(4412):971-3. doi: 10.1038/173971a0.
5
Motor activity and mitotic spindle localization of the Drosophila kinesin-like protein KLP61F.果蝇类驱动蛋白KLP61F的运动活性与有丝分裂纺锤体定位
Mol Biol Cell. 1995 Nov;6(11):1563-74. doi: 10.1091/mbc.6.11.1563.
6
Inhibition of anaphase spindle elongation in vitro by a peptide antibody that recognizes kinesin motor domain.一种识别驱动蛋白运动结构域的肽抗体在体外对后期纺锤体伸长的抑制作用。
Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6611-5. doi: 10.1073/pnas.90.14.6611.
7
Single kinesin molecules crossbridge microtubules in vitro.单个驱动蛋白分子在体外与微管形成横桥。
Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6503-7. doi: 10.1073/pnas.90.14.6503.
8
The kinesin-like protein KLP61F is essential for mitosis in Drosophila.类驱动蛋白KLP61F对果蝇的有丝分裂至关重要。
J Cell Biol. 1993 Nov;123(3):665-79. doi: 10.1083/jcb.123.3.665.
9
A "slow" homotetrameric kinesin-related motor protein purified from Drosophila embryos.从果蝇胚胎中纯化出的一种“慢速”同四聚体驱动蛋白相关运动蛋白。
J Biol Chem. 1994 Sep 16;269(37):22913-6.
10
The kinesin-related protein Eg5 associates with both interphase and spindle microtubules during Xenopus early development.在非洲爪蟾早期发育过程中，驱动蛋白相关蛋白Eg5与间期微管和纺锤体微管均有关联。
Dev Biol. 1994 Jul;164(1):147-59. doi: 10.1006/dbio.1994.1187.
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