流感血凝素与两种不同单克隆抗体的Fab片段复合物的结晶及初步X射线衍射研究
Crystallization and preliminary X-ray diffraction studies of complexes between an influenza hemagglutinin and Fab fragments of two different monoclonal antibodies.
作者信息
Gigant B, Fleury D, Bizebard T, Skehel J J, Knossow M
机构信息
Laboratoire de Biologie Structurale, UMR 9920, CNRS-Université Paris-Sud, C.N.R.S. 91198, Gif-sur-Yvette, France.
出版信息
Proteins. 1995 Sep;23(1):115-7. doi: 10.1002/prot.340230113.
PMID:8539243
Abstract
Fab fragments from two different monoclonal antibodies (BH151 and HC45) which bind to the same antigenic region of the influenza hemagglutinin were crystallized as complexes with the hemagglutinin. The complexes crystallize in PEG 600, pH 6.0, and PEG 2000, pH 8.5, respectively. Both crystals belong to space group P321, with very similar unit cell dimensions.
摘要
两种不同的单克隆抗体（BH151和HC45）的Fab片段与流感血凝素的相同抗原区域结合，并与血凝素形成复合物结晶。这些复合物分别在pH值为6.0的聚乙二醇600和pH值为8.5的聚乙二醇2000中结晶。两种晶体都属于空间群P321，晶胞尺寸非常相似。
Zotero 插件