Singh I P, Chopra A K, Coppenhaver D H, Smith E, Poast J, Baron S
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston 77555-1019, USA.
Antiviral Res. 1995 Aug;27(4):375-88. doi: 10.1016/0166-3542(95)00021-d.
Brain tissue extracts from vertebrates were examined for non-specific, broad-spectrum virus inhibitors, previously identified and characterized from other body tissues and fluids. An antiviral activity found in human, bovine, ovine, porcine, lapine, murine and piscine brain tissues shares some properties with a contact blocking-virus inhibitor, which was previously found only in cell culture supernatants. The inhibitor was active against (in order of sensitivity to inhibitor) Banzi, Sindbis, Bunyamwera, Newcastle disease, herpes simplex I, Semliki forest, polio I, mengo, vaccinia and vesicular stomatitis viruses. It is approximately 4000 kDa and possesses a complex structure containing protein, carbohydrate and lipid moieties. The inhibitor does not directly neutralize virus or induce an antiviral state in cells, but appears to act early in the replication cycle, most likely by preventing virus attachment to target cells. Its occurrence in concentrations sufficient to reduce virus yield in cell cultures at least 30-fold may indicate a role in limiting viral infections of the central nervous system.
对脊椎动物的脑组织提取物进行了检测，以寻找先前在其他身体组织和体液中鉴定并表征的非特异性、广谱病毒抑制剂。在人、牛、羊、猪、兔、鼠和鱼的脑组织中发现的一种抗病毒活性与一种接触阻断病毒抑制剂具有一些共同特性，该抑制剂先前仅在细胞培养上清液中发现。该抑制剂对（按对抑制剂的敏感性排序）班齐病毒、辛德毕斯病毒、布尼亚姆韦拉病毒、新城疫病毒、单纯疱疹病毒I型、塞姆利基森林病毒、脊髓灰质炎病毒I型、门戈病毒、痘苗病毒和水疱性口炎病毒具有活性。它的分子量约为4000 kDa，具有包含蛋白质、碳水化合物和脂质部分的复杂结构。该抑制剂不会直接中和病毒或在细胞中诱导抗病毒状态，但似乎在复制周期早期起作用，最有可能是通过阻止病毒附着于靶细胞。其在细胞培养物中以足以使病毒产量降低至少30倍的浓度出现，这可能表明它在限制中枢神经系统的病毒感染中发挥作用。
