Brown M, Gogia P P, Sinacore D R, Menton D N
Program in Physical Therapy, Washington University School of Medicine, St. Louis, MO 63108, USA.
Arch Phys Med Rehabil. 1995 Dec;76(12):1134-7. doi: 10.1016/s0003-9993(95)80122-7.
The purpose of this investigation was to determine the effects of high-voltage stimulation (HVS) on wound tensile strength properties and wound closure (histology). Eighteen mature guinea pigs with full-thickness incisions were treated with HVS for 45 minutes daily for 2 weeks; 9 animals were studied after the 14 days of treatment and the remaining 9 were studied 2 weeks later. Five animals (10 wounds) served as controls at each time period. After 2 or 4 weeks, treated and untreated skin was harvested, tested to failure, and prepared for histological examination. Two-week-treated and control wounds had comparable values for peak force to failure, elongation, and energy absorbed to failure. Epithelialization was more advanced in treated animals at 14 days (p < .05). There was a trend (p = .068) toward stronger wounds in 4-week-treated animals (maximum load to failure), but not differences were observed between controls and treated groups for elongation or energy absorbed to failure. Dermal healing appeared to be more advanced in treated animals at 30 days. Although peak force to failure was almost 500g higher for treated guinea pigs after 2 weeks of treatment and more than 700g higher than controls after 4 weeks, mean data were highly variable, so the hypothesis that HVS augments wound strength could not be accepted. It is difficult, however, not to assign clinical significance to the findings.
本研究的目的是确定高压刺激（HVS）对伤口抗张强度特性和伤口闭合（组织学）的影响。18只具有全层切口的成年豚鼠每天接受45分钟的HVS治疗，持续2周；14天治疗期结束后对9只动物进行研究，其余9只在2周后进行研究。在每个时间段，5只动物（10个伤口）作为对照。2周或4周后，采集处理过和未处理过的皮肤，测试至失效，并准备进行组织学检查。接受2周治疗的伤口和对照伤口在峰值破坏力、伸长率和吸收的破坏能量方面具有可比的值。在14天时，接受治疗的动物上皮化进程更先进（p <.05）。在接受4周治疗的动物中，伤口有更强的趋势（p = 0.068）（最大破坏负荷），但在伸长率或吸收的破坏能量方面，对照组和治疗组之间未观察到差异。在30天时，接受治疗的动物真皮愈合似乎更先进。尽管治疗2周后，治疗组豚鼠的峰值破坏力比对照组高近500克，4周后比对照组高700多克，但平均数据变化很大，因此高压刺激增强伤口强度的假设不能被接受。然而，很难不赋予这些发现临床意义。
