霍乱毒素对非洲绿猴肾细胞的促细胞增殖和细胞毒性作用及其与聚合酶链反应的关系

[Cytotonic and cytotoxic effect of cholera toxin on Vero cells and its relation to PCR].

作者信息

Rodríguez-Angeles M G, Giono-Cerezo S, Valdespino-Gómez J L

机构信息

Instituto Nacional de Diagnóstico y Referencia Epidemiológicos, México, D.F., Mexico.

出版信息

Rev Latinoam Microbiol. 1994 Oct-Dec;36(4):263-71.

PMID:7701136

Abstract

We studied 40 Vibrio cholerae strains: 16 from stool, 16 from sewage and 8 from food. The serotypes were Inaba in 21 strains, 8 Ogawa strains and 11 V. cholerae non-O1. PCR was made with ctx2 and ctx3 primers with 25 cycles of temperature: 1 min at 94 degrees C, 1 min at 60 degrees C and 1 min at 72 degrees C. 24 V. cholerae strains were positive: 18/24 Inaba y 6/24 Ogawa. PCR was negative for 16 strains: 3 Inaba serotype, 2 Ogawa y 11 V. cholerae non-O1. In Vero culture cells 18 strains were cytotonic, 21 cytotoxic and 1 strain was negative. ELISA was positive for 11 strains with PCR positive. The PCR sensitivity was 95.83% compared with culture cells. V. cholerae O1 produced cytotoxic effect on Vero culture cells, maybe related to ACE factor. Colony blot was made with a specific probe labeled with digoxigenin and it could detect 4 Vibrio cholerae toxigenic strains with PCR negative. All V. cholerae Non O1 strains were PCR negative.

摘要

我们研究了40株霍乱弧菌菌株：16株来自粪便，16株来自污水，8株来自食物。血清型为稻叶型的有21株，小川型8株，非O1群霍乱弧菌11株。使用ctx2和ctx3引物进行PCR，温度循环25次：94℃ 1分钟，60℃ 1分钟，72℃ 1分钟。24株霍乱弧菌菌株呈阳性：稻叶型18/24，小川型6/24。16株菌株PCR呈阴性：稻叶血清型3株，小川型2株，非O1群霍乱弧菌11株。在Vero培养细胞中，18株具有细胞膨胀性，21株具有细胞毒性，1株呈阴性。11株PCR阳性的菌株ELISA呈阳性。与培养细胞相比，PCR敏感性为95.83%。O1群霍乱弧菌对Vero培养细胞产生细胞毒性作用，可能与ACE因子有关。用洋地黄毒苷标记的特异性探针进行菌落杂交，可检测出4株PCR阴性的产毒霍乱弧菌菌株。所有非O1群霍乱弧菌菌株PCR均为阴性。