Tume C, Sun E, Titanji V, Bathurst I, Barr P, Hough M, Weiss N, Chakravarti B, Wahi M, Lackey A
Department of Pathology, University of California, San Francisco, USA.
Exp Parasitol. 1995 Dec;81(4):445-52. doi: 10.1006/expr.1995.1137.
A major antigen recognized by human sera in Onchocerca volvulus infections is a parasite eggshell protein. The cDNA clone for this antigen was isolated from a lambda gt11 O. volvulus cDNA library using antisera from patients with high microfilarial counts. Sequence analysis of the cDNA clone predicts a polyglutamine repeat near the 5' end of the cDNA, and a motif of four arginines near the 3' end, reminiscent of that found in many regulatory proteins. The cDNA was subcloned into a yeast expression vector and reagent quantities of recombinant antigen produced in Saccharomyces cerevisiae. Antisera produced to the recombinant purified protein localized the antigen to the eggshell of developing microfilariae within the adult female uterus. No other sites of Oveg1 expression were noted in adult worms, but labeling was seen in internal membrane structures of L3 larvae. Sera from infected chimps recognized Oveg1 only after infections became patent. Sera from infected humans showed reactivity to Oveg1 that varied from 39 to 95%, depending upon the geographic location.
人体血清在盘尾丝虫感染中识别的一种主要抗原是一种寄生虫卵壳蛋白。使用来自微丝蚴计数高的患者的抗血清，从λgt11盘尾丝虫cDNA文库中分离出该抗原的cDNA克隆。对该cDNA克隆的序列分析预测，在cDNA的5'端附近有一个聚谷氨酰胺重复序列，在3'端附近有一个由四个精氨酸组成的基序，这让人联想到在许多调节蛋白中发现的基序。将该cDNA亚克隆到酵母表达载体中，并在酿酒酵母中产生试剂级别的重组抗原。针对重组纯化蛋白产生的抗血清将该抗原定位到成年雌性子宫内发育中的微丝蚴的卵壳上。在成年蠕虫中未发现Oveg1表达的其他位点，但在L3幼虫的内膜结构中可见标记。感染黑猩猩的血清只有在感染变得明显后才识别Oveg1。来自受感染人类的血清对Oveg1的反应性从39%到95%不等，这取决于地理位置。
