Haoudi A, Kim M H, Champion S, Best-Belpomme M, Maisonhaute C
Laboratoire de Genetique Cellulaire et Moleculaire, UA 1135 CNRS, Université Pierre and Marie Curie, Paris, France.
FEBS Lett. 1995 Dec 11;377(1):67-72. doi: 10.1016/0014-5793(95)01305-9.
1731 is a Drosophila melanogaster retrotransposon whose nucleotide sequence shows a proviral architecture with two long terminal repeats (LTRs) framing two internal Open Reading Frames (ORFs). The pol ORF2 of this mobile genetic element was demonstrated to code for an active Reverse Transcriptase (RT) and the ORF1 is expected to code for the structural Gag proteins of the virus-like particles (VLP). Using specific anti-Gag antibodies, we have characterized the 1731 Gag polypeptides expressed either in vitro or in Kc Drosophila melanogaster cultured cells. Together with the 1731 RT, the largest, likely post-translationaly-modified Gag polypeptides are gathered into cytoplasmic virus-like particles. Moreover and consistent with the nuclear localization signal present in the Gag sequence, we observed that a short 1731 Gag polypeptide is associated to the cell nuclei.
1731是一种黑腹果蝇逆转座子，其核苷酸序列显示出一种前病毒结构，有两个长末端重复序列（LTR）构成两个内部开放阅读框（ORF）。已证明这种移动遗传元件的pol ORF2编码一种活性逆转录酶（RT），并且预计ORF1编码病毒样颗粒（VLP）的结构Gag蛋白。使用特异性抗Gag抗体，我们对在体外或黑腹果蝇Kc培养细胞中表达的1731 Gag多肽进行了表征。与1731 RT一起，最大的、可能经过翻译后修饰的Gag多肽聚集形成细胞质病毒样颗粒。此外，与Gag序列中存在的核定位信号一致，我们观察到一种短的1731 Gag多肽与细胞核相关。
